England will be without Immanuel Feyi-Waboso and Tom Curry for the remainder of the Nations Championship after both players were ruled out through injury.
It comes after Steve Borthwick’s side have been hit with a string of injuries ahead of their game against Japan on Saturday.
Seb Atkinson, who came off in the second half of England’s 45-22 win against Australia, will miss the game with a groin injury, along with Ollie Chessum (shoulder) and George Furbank (groin).
England are optimistic that the trio will be available for their clash against New Zealand at Allianz Stadium on Saturday, November 21.
Feyi-Waboso suffered an injury to his right ankle during the first half of England’s clash against the Wallabies after being tackled by Australia prop Angus Bell.
Bell was later sent to the sin bin, with Henry Pollock coming on to replace Feyi-Waboso on the wing at half-time. Pollock would go on to score twice.
The Exeter winger’s absence could see Borthwick call up Saracens fliers Noah Caluori or Tobias Elliott for England’s clash with Japan.
Tom Curry missed England’s game against Australia with a thigh injury he picked up in training
This would be Caluori’s third cap for England, having made his senior debut during their Nations Championship match against Fiji in July.
Centers Joe Marchant and Will Joseph could also be considered as wing options, while Fraser Dingwall or Max Ojomoh are likely to take the No 12 shirt in place of Atkinson.
Curry did not feature in England’s win over Australia after picking up a groin injury in training last week.
Borthwick revealed after Sunday’s win that his brother Ben Curry was unlikely to start against Japan, despite the opening side enjoying a fine outing against the Wallabies.
Borthwick is likely to deploy Freddie Steward at full-back in place of Furbank and keep Marcus Smith on the bench as utility cover.
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Steve Borthwick hails Henry Pollock ‘brilliant’ after a standout performance in the wing for England against Australia
England’s 36-man squad returned to Pennyhill Park on Monday to prepare for this weekend’s game, with Jack Kenningham and Max Hicks both drafted into the squad as back-up for Ben Curry and Chessum.
Meanwhile, Archie McParland has returned to the training squad after recovering from a calf injury.
England training squad
Attacker:
Fin Baxter (Harlequins)
Arthur Clark (Gloucester Rugby)
Alex Coles (Northampton Saints)
Luke Cowan-Dickie (Sale Sharks)
Ben Curry (Sale Sharks)
Theo Dan (Saracens)
Ben Earl (Saracens)
Greg Fisilau (Exeter Chiefs)
Jamie George (Saracens)
Ellis Genge (Bristol Bears)
Joe Heyes (Leicester Tigers)
Max Hicks (Newcastle Red Bulls)
Maro Itoje (Saracens)
Emmanuel Iyogun (Northampton Saints)
Jack Kenningham (Harlequins)
George Martin (Saracens)
Asher Opoku-Fordjour (Sale Sharks)
Tom Pearson (Northampton Saints)
Henry Pollock (Northampton Saints)
Will Stuart (Bathroom Rugby)
Back:
Noah Caluori (Saracens)
Fraser Dingwall (Northampton Saints)
Tobias Elliott (Saracens)
George Ford (Sale Sharks)
Tommy Freeman (Northampton Saints)
Will Joseph (Gloucester Rugby)
Joe Marchant (Sale Sharks)
Aden Murley (Harlequins)
Archie McParland (Northampton Saints)
Max Ojomoh (Rugby Bath)
Henry Slade (Exeter Chiefs)
Fin Smith (Northampton Saints)
Marcus Smith (Harlequins)
Ben Spencer (Rugby Bath)
Freddie Steward (Leicester Tigers)
Jack van Poortvliet (Leicester Tigers)
Rehabilitation: Seb Atkinson (Gloucester Rugby), Ollie Chessum (Leicester Tigers), George Furbank (Harlequins)